TNF-alpha Protein, Mouse, Recombinant

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TNF-alpha Protein, Mouse, Recombinant: Product Information

Purity
> 98 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
Protein Construction
A DNA sequence encoding the soluble form of mouse TNF-α (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the C-terminus.
Accession#
Expressed Host
E. coli
Species
Mouse
Predicted N Terminal
Met
Molecule Mass
The recombinant mouse TNF-α consists of 157 amino acids and migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 50mM Tris 0.2M NaCl, pH 7.2
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNF-alpha Protein, Mouse, Recombinant: Images

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.

TNF-alpha Protein, Mouse, Recombinant: Alternative Names

DIF Protein, Mouse; TNF-a Protein, Mouse; TNF-alpha Protein, Mouse; Tnfa Protein, Mouse; TNFalpha Protein, Mouse; Tnfsf1a Protein, Mouse; TNFSF2 Protein, Mouse

TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Full Name
tumor necrosis factor
Research Areas
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Sharpin contributes to TNFα dependent NFκB activation and anti-apoptotic signalling in hepatocytes
    Author
    Sieber, S;Lange, N;Kollmorgen, G;Erhardt, A;Quaas, A;Gontarewicz, A;Sass, G;Tiegs, G;Kreienkamp, HJ;
    Year
    2012
    Journal
    PLoS ONE
    Application
    cell-based (induction)
  • Sharpin Controls Osteogenic Differentiation of Mesenchymal Bone Marrow Cells
    Author
    Jeschke, A;Catala-Lehnen, P;Sieber, S;Bickert, T;Schweizer, M;Koehne, T;Wintges, K;Marshall, RP;Mautner, A;Duchstein, L;Otto, B;Horst, AK;Amling, M;Kreienkamp, HJ;Schinke, T;
    Year
    2015
    Journal
    J. Immunol.
    Application
    cell culture
  • Angiopoietin-Like Protein 7 Promotes an Inflammatory Phenotype in RAW264.7 Macrophages Through the P38 MAPK Signaling Pathway
    Author
    Qian, T;Wang, K;Cui, J;He, Y;Yang, Z;
    Year
    2016
    Journal
    Inflammation
    Application
    animal-based
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