Anti-CD4 rabbit polyclonal antibody at 1:500 dilution
Lane A: THP1 Whole Cell Lysate
Lane B: Mouse slpeen tissue lysateLysates/proteins at 30 μg per laneSecondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:51 kDa
Observed band size:58 kDa
mCD4 was immunoprecipitated using:
Lane A:0.5 mg THP1 Whole Cell Lysate
0.5 µL anti-mCD4 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Anti-mCD4 rabbit polyclonal antibody,at 1:500 dilution
Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size :51 kDa