Anti-ABCF2 Antibody, Rabbit Polyclonal

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Anti-ABCF2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-ABCF2 Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF,IP
Species reactivity
Reacts with: Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Specificity
Human ABCF2
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human ABCF2
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ABCF2, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ABCF2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:500-1:2500
IP IP:0.1-0.5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ABCF2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-ABCF2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HeLa Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: HepG2 Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:71 kDa

Observed band size:71 kDa

Immunofluorescence staining of ABCF2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ABCF2 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

ABCF2 was immunoprecipitated using:

Lane A:0.5 mg HeLa Whole Cell Lysate

1 µL anti-ABCF2 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-ABCF2 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 71 kDa

Observed band size :71 kDa

Anti-ABCF2 Antibody, Rabbit Polyclonal: Synonyms

Anti-ABC28 Antibody; Anti-EST133090 Antibody; Anti-HUSSY-18 Antibody; Anti-HUSSY18 Antibody

ABCF2 Background Information

Full Name
ATP-binding cassette, sub-family F (GCN20), member 2

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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