ASL was immunoprecipitated using:
Lane A:0.5 mg HeLa Whole Cell Lysate
4 µL anti-ASL rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Anti-ASL rabbit polyclonal antibody,at 1:100 dilution
Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size :54 kDa