Anti-Argininosuccinate lyase Antibody, Rabbit Polyclonal

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Anti-Argininosuccinate lyase Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-Argininosuccinate lyase Antibody, Rabbit Polyclonal
Validated applications
IP
Species reactivity
Reacts with: Human
Predicted to React with: Mouse, Rat, Cynomolgus (Species predicted to react based on 100% sequence homology)
Specificity
Human Argininosuccinate lyase
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human ASL
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ASL, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Argininosuccinate lyase Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
IP IP:5-10 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Argininosuccinate lyase Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

ASL was immunoprecipitated using:

Lane A:0.5 mg HeLa Whole Cell Lysate

4 µL anti-ASL rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-ASL rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 51 kDa

Observed band size :54 kDa

Anti-Argininosuccinate lyase Antibody, Rabbit Polyclonal: Synonyms

Anti-ASAL Antibody; Anti-ASL Antibody

Argininosuccinate lyase Background Information

The recycling of citrulline by argininosuccinate synthase 1 (ASS1) and argininosuccinate lyase (ASL) is crucial to maintain arginine availability and nitric oxide (NO) production. Nitric oxide (NO) plays an established role in numerous physiological and pathological processes, but the specific cellular sources of NO in disease pathogenesis remain unclear, preventing the implementation of NO-related therapy. Argininosuccinate lyase (ASL) is the only enzyme able to produce arginine, the substrate for NO generation by nitric oxide synthase (NOS) isoforms. Induction of endogenous NO production by enterocytes with supplements that upregulate ASL expression and complement its substrates results in improved epithelial integrity and alleviation of colitis and of inflammation-associated colon cancer.
Full Name
argininosuccinate lyase

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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