Anti-ASB9 Antibody, Rabbit Polyclonal

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Anti-ASB9 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-ASB9 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human ASB9
Immunogen
E. coli-derived Human ASB9 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human ASB9 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-ASB9 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:500-1:2000
ICC-IF 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ASB9 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-ASB9 rabbit polyclonal antibody at 1:500 dilution

Lane A: U-251 MG Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lane C: MOLT4 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:32 kDa

Observed band size:32 kDa

(We are unsure as to the identity of these extra bands.)

Immunochemical staining of human ASB9 in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of ASB9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ASB9 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

ASB9 Background Information

Full Name
ankyrin repeat and SOCS box containing 9

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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