Anti-BBX Antibody, Rabbit Polyclonal

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Anti-BBX Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-BBX Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human BBX
Immunogen
E. coli-derived Human BBX fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human BBX fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-BBX Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:500-1:2500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-BBX Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-BBX rabbit polyclonal antibody at 1:500 dilution

Lane A: U-251 MG Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:102 kDa

Observed band size:102 kDa

Immunofluorescence staining of BBX in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human BBX polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Anti-BBX Antibody, Rabbit Polyclonal: Synonyms

Anti-ARTC1 Antibody; Anti-HBP2 Antibody; Anti-HSPC339 Antibody; Anti-MDS001 Antibody

BBX Background Information

Full Name
bobby sox homolog (Drosophila)

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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