Anti-BLBP Antibody, Rabbit Polyclonal

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Anti-BLBP Antibody, Rabbit Polyclonal General Information

Product name
Anti-BLBP Antibody, Rabbit Polyclonal
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human BLBP
Immunogen
Recombinant Human BLBP/FABP7 Protein (Catalog#12577-HNAE)
Preparation
Produced in rabbits immunized with purified, recombinant Human BLBP/FABP7 (rh BLBP/FABP7; Catalog#12577-HNAE; O15540; Met1-Ala132). BLBP/FABP7 specific IgG was purified by Human BLBP/FABP7 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-BLBP Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:1000-1:10000
IP IP:0.5-2μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-BLBP Antibody, Rabbit Polyclonal Images

Anti-FABP7 rabbit polyclonal antibody at 1:500 dilution

Lane A: U-251 MG Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:15 kDa

Immunochemical staining of human FABP7 in human malignant melanoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human FABP7 in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

FABP7 was immunoprecipitated using:

Lane A:0.5 mg U251MG Whole Cell Lysate

4 µL anti-FABP7 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-FABP7 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 15 kDa

Observed band size :15 kDa

Anti-BLBP Antibody, Rabbit Polyclonal: Synonyms

Anti-B-FABP Antibody; Anti-BLBP Antibody; Anti-FABPB Antibody; Anti-LTR2-FABP7 Antibody; Anti-MRG Antibody; Anti-RP11-548E19.1 Antibody

BLBP Background Information

BLBP, also known as FABP7, is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP7 binds DHA with the highest affinity among all of the FABPs. FABPs may play roles in fatty acid uptake, transport, and metabolism. BLBP is expressed, during development, in radial glia by the activation of notch receptors. It was shown that reelin induces FABP7 expression in neural progenitor cells via notch-1 activation. BLBP variation is linked to weak prepulse inhibition(PPI) in mice and deficit in PPI is an endophenotypic trait observed in schizophrenia patients and their relatives.
Full Name
fatty acid binding protein 7, brain
References
  • Shi YE, et al. (1997) Antitumor activity of the novel human breast cancer growth inhibitor, mammary-derived growth inhibitor-related gene, MRG. Cancer Res. 57(15):3084-91.
  • Young JK, et al. (1997) Immunoreactivity for brain-fatty acid binding protein in gomori-positive astrocytes. Glia. 16(3):218-26.
  • Xu LZ, et al. (1996) Ligand specificity of brain lipid-binding protein. J Biol Chem. 271(40): 24711-9.

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