SARS-CoV Spike S1 Subunit Antibody, Mouse MAb

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SARS-CoV Spike S1 Subunit Antibody, Mouse MAb General Information

Product name
SARS-CoV Spike S1 Subunit Antibody, Mouse MAb
Validated applications
WB,ELISA
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS Coronavirus spike
Has cross-reactivity in ELISA and WB with
SARS-CoV Spike S1 (Cat# 40150-V08B1)
No cross-reactivity in ELISA and WB with
SARS-CoV Spike RBD (Cat# 40150-V08B2)
SARS-CoV-2 (2019-nCoV) Spike S1 (Cat# 40591-V08H1)
SARS-CoV-2 (2019-nCoV) Spike RBD (Cat# 40592-V08B)
Immunogen
Recombinant SARS-CoV Spike S1 Subunit Protein (Catalog#40150-V08B1)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV Spike S1 Subunit ( Catalog#40150-V08B1; AAX16192.1; Met1-Arg667). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2a Clone #08
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV Spike S1 Subunit Antibody, Mouse MAb Validated Applications

Application Dilution
WB 1:1000-1:5000
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV Spike S1 Subunit Antibody, Mouse MAb Images

Anti-SARS-COV S1 mouse monoclonal  antibody at 1:1000 dilution.

Lane A:  SARS-CoV Spike S1 (Cat# 40150-V08B1) (30ng)

Lane B:  SARS-CoV Spike S1 (Cat# 40150-V08B1) (5ng)

Secondary

Goat Anti-Mouse IgG (H+L)/HRP  at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Citation List

SARS-CoV Spike S1 Subunit Antibody, Mouse MAb: Synonyms

Anti-coronavirus s1 Antibody; Anti-coronavirus s2 Antibody; Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov s1 Antibody; Anti-ncov s2 Antibody; Anti-ncov spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus s1 Antibody; Anti-novel coronavirus s2 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-s2 Antibody; Anti-Spike RBD Antibody

Coronavirus spike Background Information

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
References
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

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