SARS-CoV Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified

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SARS-CoV Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified General Information

Product name
SARS-CoV Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified
Validated applications
WB,ELISA
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS Coronavirus spike
Immunogen
Recombinant SARS-CoV Spike S1 Subunit protein (Catalog#40150-V08B1)
Preparation
Produced in rabbits immunized with purified, recombinant SARS-CoV Spike S1 Subunit (Catalog#40150-V08B1; AAX16192.1; Met1-Arg667). SARS-CoV Spike S1 Subunit specific IgG was purified by SARS-CoV Spike S1 Subunit affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified Images

Anti-Human SARS Coronavirus Spike S1 Subunit rabbit polyclonal antibody at 1:1000 dilution

Sample:Human SARS Coronavirus Spike S1 Subunit Recombinant Protein

Lane A: 30ng

Secondary

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/1000 dilution.

Performed under reducing conditions.

Citation List

SARS-CoV Spike S1 Subunit Antibody, Rabbit PAb, Antigen Affinity Purified: Synonyms

Anti-coronavirus s1 Antibody; Anti-coronavirus s2 Antibody; Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov s1 Antibody; Anti-ncov s2 Antibody; Anti-ncov spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus s1 Antibody; Anti-novel coronavirus s2 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-s2 Antibody; Anti-Spike RBD Antibody

Coronavirus spike Background Information

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
References
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
  • Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV
    Author
    Ou, X;Liu, Y;Lei, X;Li, P;Mi, D;Ren, L;Guo, L;Guo, R;Chen, T;Hu, J;Xiang, Z;Mu, Z;Chen, X;Chen, J;Hu, K;Jin, Q;Wang, J;Qian, Z;
    Year
    2020
    Journal
    Nat Commun
  • COVID-19 Pandemic: Insights into Structure, Function, and hACE2 Receptor Recognition by the SARS-CoV-2
    Author
    Mittal, A;Manjunath, K;Ranjan, R;Kaushik, S;Kumar, S;Verma, V;
    Year
    2020
    Journal
    preprints
    Application
    binding
  • Development of a Synthetic Poxvirus-Based SARS-CoV-2 Vaccine
    Author
    Chiuppesi, F;Salazar, MD;Contreras, H;Nguyen, VH;Martinez, J;Park, S;Nguyen, J;Kha, M;Iniguez, A;Zhou, Q;Kaltcheva, T;Levytskyy, R;Ebelt, ND;Kang, TH;Wu, X;Rogers, T;Manuel, ER;Shostak, Y;Diamond, DJ;Wussow, F;
    Year
    2020
    Journal
    bioRxiv
    Application
    WB
  • Human angiotensin-converting enzyme 2 transgenic mice infected with SARS-CoV-2 develop severe and fatal respiratory disease
    Author
    Golden, J;Cline, C;Zeng, X;Garrison, A;Carey, B;Mucker, E;White, L;Shamblin, J;Brocato, R;Liu, J;Babka, A;Rauch, H;Smith, J;Hollidge, B;Fitzpatrick, C;Badger, C;Hooper, J;
    Year
    2020
    Journal
    bioRxiv
    Application
    IF;
  • A single-dose live-attenuated YF17D-vectored SARS-CoV2 vaccine candidate
    Author
    Felipe, L;Vercruysse, T;Sharma, S;Ma, J;Lemmens, V;van Looveren, D;Arkalagud Javarappa, M;Boudewijns, R;Malengier-Devlies, B;Kaptein, S;Liesenborghs, L;De Keyzer, C;Bervoets, L;Rasulova, M;Seldeslachts, L;Jansen, S;Yakass, M;Quaye, O;Li, L;Zhang, X;Horst, S;Mishra, N;Coelmont, L;Cawthorne, C;Van Laere, K;Opdenakker, G;Van de Velde, G;Weynand, B;Teuwen, D;Matthys, P;Neyts, J;Thibaut, H;Dallmeier, K;
    Year
    2020
    Journal
    bioRxiv
    Application
    WB

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