|IP||1-4 μL/mg of lysate|
|ELISA(Cap)：||This antibody will detect Human 2B4 / CD244 in ELISA pair set (Catalog: # SEK10042). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10042-R031).|
Anti-2B4 rabbit monoclonal antibody at 1:500 dilution
Lane A: Daudi Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:42 kDa
Observed band size:28 kDa
(We are unsure as to the identity of these extra bands.)
2B4 was immunoprecipitated using:
Lane A:0.5 mg Daudi Whole Cell Lysate2 µL anti-2B4 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-2B4 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 42 kDa
Observed band size: 28 kDa