Anti-ADAM17 Antibody, Rabbit Polyclonal

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Anti-ADAM17 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-ADAM17 Antibody, Rabbit Polyclonal
Validated applications
IP
Species reactivity
Reacts with: Human
Specificity
Human ADAM17
Immunogen
A synthetic peptide corresponding to the C-terminus of the human CD156b
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human CD156b, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ADAM17 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ADAM17 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

CD156b was immunoprecipitated using:

Lane A:0.5 mg Raw264.7 Whole Cell Lysate

2 µL anti-CD156b rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD156b rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 93 kDa

Observed band size: 80 kDa

Anti-ADAM17 Antibody, Rabbit Polyclonal: Synonyms

Anti-ADAM18 Antibody; Anti-CD156B Antibody; Anti-CSVP Antibody; Anti-NISBD Antibody; Anti-NISBD1 Antibody; Anti-TACE Antibody

ADAM17 Background Information

ADAM17 is a member of the ADAM protein family of disintegrins and metalloproteases. ADAM17 is ubiquitously expressed in the human colon, with increased activity in the colonic mucosa of patients with ulcerative colitis, a main form of inflammatory bowel disease. The expression of ADAM17 may be inhibited by ethanol. It is involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. ADAM17 also plays a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes. ADAM17 may play a prominent role in the Notch signaling pathway, during the proteolytic release of the Notch intracellular domain (from the Notch1 receptor) that occurs following ligand binding.
Full Name
ADAM metallopeptidase domain 17
Related Pathways
  • Notch Signaling
    Notch Signaling
References
  • Poghosyan. et al., 2002, J Biol Chem. 277 (7): 4999-5007.
  • Li Y. et al., 2006, Blood. 108 (7): 2275-9.
  • Peiretti. et al., 2003, J Cell Sci. 116 (10): 1949-57.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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