Recombinant Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Monoclonal

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Recombinant Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) General Information

Product name
Recombinant Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Monoclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human Alkaline Phosphatase/ALPL
Immunogen
A synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase / ALPL
Preparation
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase / ALPL. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Rabbit IgG Clone #077
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) Images

ALPL was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-ALPL rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ALPL rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 57 kDa

Observed band size: 86 kDa

Anti-ALPL rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:57 kDa

Observed band size:95 kDa

Anti-ALPL rabbit monoclonal antibody at 1:500 dilution

Lane A: ALPL konckout Hela Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:57 kDa

Observed band size:95 kDa

Recombinant Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Monoclonal: Synonyms

Anti-AP-TNAP Antibody; Anti-APTNAP Antibody; Anti-HOPS Antibody; Anti-TNAP Antibody; Anti-TNSALP Antibody

Alkaline Phosphatase/ALPL Background Information

Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.
Full Name
alkaline phosphatase, liver/bone/kidney
References
  • Brun-Heath I, et al. (2011) Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues. Cell Tissue Res. 343(3): 521-36.
  • Whyte MP, et al. (1995) Alkaline phosphatase: placental and tissue-non-specific isoenzymes hydrolyze phosphoethanolamine, inorganic pyrophosphate, and pyridoxal 5-phosphate. J Clin Invest. 95: 1440-5.
  • Whyte MP. (1994) Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. Endocrinol Rev. 4: 439-61.
  • Weinreb M, et al. (1990) Different pattern of alkaline phosphatase, osteopontin and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res. 5: 831-42.

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