Anti-BCRP/ABCG2 Antibody, Rabbit Polyclonal

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Anti-BCRP/ABCG2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-BCRP/ABCG2 Antibody, Rabbit Polyclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human BCRP/ABCG2
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human ABCG2
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ABCG2, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-BCRP/ABCG2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-BCRP/ABCG2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

ABCG2 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-ABCG2 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-ABCG2 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 70 kDa

Observed band size: 70 kDa

Anti-ABCG2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: cos-7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:72 kDa

Observed band size:70 kDa

Anti-BCRP/ABCG2 Antibody, Rabbit Polyclonal: Synonyms

Anti-ABC15 Antibody; Anti-ABCP Antibody; Anti-BCRP Antibody; Anti-BCRP1 Antibody; Anti-BMDP Antibody; Anti-CD338 Antibody; Anti-CDw338 Antibody; Anti-EST157481 Antibody; Anti-GOUT1 Antibody; Anti-MRX Antibody; Anti-MXR Antibody; Anti-MXR1 Antibody; Anti-UAQTL1 Antibody

BCRP/ABCG2 Background Information

ATP-binding cassette subfamily G member 2 (ABCG2), a member of the ABC transporter superfamily proteins. the expression of ABCG2 is related to tumorigenic cancer stem cells (CSC) in several cancers, mediates multidrug resistance (MDR) by transporting substrate anticancer drugs out of cancer cells and decreasing their intracellular accumulation. ABCB1 and ABCG2 are efflux transporters which have a major impact on the pharmacological behavior of numerous drugs. They are expressed in the intestine, liver, kidney, BBB and placenta. It has become evident that ABCB1 and ABCG2 modify the pharmaco/toxicokinetics in the placenta and fetus and may consequently affect the outcome of pregnancy.
Full Name
ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group)
Research Areas

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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