CCR1/CD191 was immunoprecipitated using:
Lane A:0.5 mg Jurkat Whole Cell Lysate
Lane B:0.5 mg 293T Whole Cell Lysate4 µL anti-CCR1/CD191 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:
Anti-CCR1/CD191 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 43 kDa
Observed band size: 43 kDa
Anti-CCR1/CD191 rabbit polyclonal antibody at 1:500 dilution.
Lane A: HEK293 Membrane Lysate
Lane B: 293T Membrane LysateLysates/proteins at 30 μg per lane.
Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:43 kDa
Observed band size:43 kDa