Anti-CCR1 Antibody, Rabbit Polyclonal

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Anti-CCR1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-CCR1 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human CCR1
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human CCR1/CD191
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human CCR1/CD191, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CCR1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:500-1:2000
ICC-IF 1:300-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CCR1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Immunofluorescence staining of CCR1/CD191 in HESS9 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CCR1/CD191 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cell membrane.
Immunochemical staining of human CCR1/CD191 in human tonsil with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human CCR1/CD191 in human spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

CCR1/CD191 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

4 µL anti-CCR1/CD191 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-CCR1/CD191 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 43 kDa

Observed band size: 43 kDa

Anti-CCR1/CD191 rabbit polyclonal antibody at 1:500 dilution.

Lane A: HEK293 Membrane Lysate

Lane B: 293T Membrane Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:43 kDa

Anti-CCR1 Antibody, Rabbit Polyclonal: Synonyms

Anti-CD191 Antibody; Anti-CKR-1 Antibody; Anti-CKR1 Antibody; Anti-CMKBR1 Antibody; Anti-HM145 Antibody; Anti-MIP1aR Antibody; Anti-SCYAR1 Antibody

CCR1 Background Information

Full Name
chemokine (C-C motif) receptor 1
Related Pathways
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    AKT Signaling Pathway
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    Jak-Stat Signaling Pathway
  • Autophagy Pathway
    Autophagy Pathway

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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