Anti-cd192 rabbit polyclonal antibody at 1:500 dilution.
Lane A: A431 Membrane LysateLysates/proteins at 30 ug per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:42 kDa
Observed band size:48 kDa
(We are unsure as to the identity of these extra bands.)
CD192 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate2 µL anti-CD192 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:
Anti-CD192 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 45 kDa
Observed band size: 45 kDa