Anti-CEBP alpha/CEBPA Antibody, Rabbit Polyclonal

1/2
Price:
Size:
Number:

Anti-CEBP alpha/CEBPA Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-CEBP alpha/CEBPA Antibody, Rabbit Polyclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Specificity
Human CEBP alpha/CEBPA
Immunogen
A synthetic peptide corresponding to the C-terminus of the human CEBPA .
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the human CEBPA . Huamn CEBPA specific IgG was purified by Huamn CEBPA affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CEBP alpha/CEBPA Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CEBP alpha/CEBPA Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

CEBPA was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-CEBPA rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CEBPA rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 38 kDa

Observed band size: 38 kDa

Anti-CEBPA rabbit polyclonal antibody at 1:500 dilution

Lane A: U937 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:37 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CEBP alpha/CEBPA Antibody, Rabbit Polyclonal: Synonyms

Anti-C/EBP-alpha Antibody; Anti-CEBP Antibody

CEBP alpha/CEBPA Background Information

The CCAAT/enhancer-binding protein-alpha (CEBPA) is lineage-specific transcription factor in the hematopoietic system. The presence of biallelic CEBPA mutations is a favourable prognostic feature in acute myeloid leukaemia (AML). CEBPA mutations are currently identified through conventional capillary sequencing (CCS). Mutations in CCAAT/enhancer binding protein alpha (CEBPA) occur in 5-1% of cases of acute myeloid leukemia. CCAAT/enhancer binding protein alpha gene (CEBPA) is an important transcription factor in maintenance of differentiation of granulocyte series of hematopoietic system. It plays a key role in regulating cell proliferation and differentiation.
Full Name
CCAAT/enhancer binding protein alpha
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.