Anti-Connexin 43/GJA1 Antibody, Rabbit Polyclonal

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Anti-Connexin 43/GJA1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-Connexin 43/GJA1 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Specificity
Human Connexin 43/GJA1
Immunogen
A synthetic peptide corresponding to the C-terminus of the human GJA1
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human GJA1 , and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Connexin 43/GJA1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IHC-P 1:5000-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Connexin 43/GJA1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Immunochemical staining of human GJA1 in mouse heart with rabbit polyclonal antibody at 1:50000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human GJA1 in human testis with rabbit polyclonal antibody at 1:50000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human GJA1 in cynomolgus heart with rabbit polyclonal antibody at 1:50000 dilution, formalin-fixed paraffin embedded sections.

Anti-GJA1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Rat brain Tissue Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:48 kDa

Anti-Connexin 43/GJA1 Antibody, Rabbit Polyclonal: Synonyms

Anti-AVSD3 Antibody; Anti-CMDR Antibody; Anti-CX43 Antibody; Anti-EKVP Antibody; Anti-EKVP3 Antibody; Anti-GJAL Antibody; Anti-HLHS1 Antibody; Anti-HSS Antibody; Anti-ODDD Antibody; Anti-PPKCA Antibody

Connexin 43/GJA1 Background Information

GJA1 encodes connexin 43 (Cx43), the most widely expressed gap junction protein. The de novo missense mutations in GJA1 (gap junction protein alpha 1) cause EKVP. Erythrokeratodermia variabilis et progressiva (EKVP) is a rare, inherited skin disease that is characterized by transient figurate patches of erythema, localized or generalized scaling, and frequent palmoplantar keratoderma. GJA1 and SERPINE2 represent potential gene markers associated with oocyte maturation. In the GJA1 gene which encodes gap junction channel protein connexin 43.
Full Name
gap junction protein, alpha 1, 43kDa

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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