ASF1B was immunoprecipitated using:
Lane A:0.5 mg K562 Whole Cell Lysate0.5 µL anti-ASF1B rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:
Anti-ASF1B rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 22 kDa
Observed band size: 22 kDa
Anti-CXCL1 rabbit polyclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:11 kDa
Observed band size:13 kDa
(We are unsure as to the identity of these extra bands.)