Anti-CXCL1 Antibody, Rabbit Polyclonal

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Anti-CXCL1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-CXCL1 Antibody, Rabbit Polyclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human CXCL1
Immunogen
A synthetic peptide corresponding to the center region of the Human CXCL1
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human CXCL1, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CXCL1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IP 0.1-0.5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CXCL1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

ASF1B was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

0.5 µL anti-ASF1B rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-ASF1B rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 22 kDa

Observed band size: 22 kDa

Anti-CXCL1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:11 kDa

Observed band size:13 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CXCL1 Antibody, Rabbit Polyclonal: Synonyms

Anti-CA21H Antibody; Anti-CAH1 Antibody; Anti-CPS1 Antibody; Anti-CYP21 Antibody; Anti-CYP21B Antibody; Anti-FSP Antibody; Anti-GRO1 Antibody; Anti-GROa Antibody; Anti-MGSA Antibody; Anti-MGSA-a Antibody; Anti-NAP-3 Antibody; Anti-P450c21B Antibody; Anti-SCYB1 Antibody

CXCL1 Background Information

The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a). CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2? in chondrocytes and support the important role of AP-2? in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.
Full Name
chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Wang JG, et al. (2008) The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons. Mol Pain. 4: 38.
  • Acosta JC, et al. (2009) A role for CXCR2 in senescence, but what about in cancer? Cancer Res. 69(6): 2167-70.
  • Onan D, et al. (2009) The chemokine Cxcl1 is a novel target gene of parathyroid hormone (PTH)/PTH-related protein in committed osteoblasts. Endocrinology. 150(5): 2244-53.
  • Ritzman AM, et al. (2010) The chemokine receptor CXCR2 ligand KC (CXCL1) mediates neutrophil recruitment and is critical for development of experimental Lyme arthritis and carditis. Infect Immun. 78(11): 4593-600.
  • Bolitho C, et al. (2010) The chemokine CXCL1 induces proliferation in epithelial ovarian cancer cells by transactivation of the epidermal growth factor receptor. Endocr Relat Cancer. 17(4): 929-40.
  • Wenke AK, et al. (2011) The transcription factor AP-2? regulates CXCL1 during cartilage development and in osteoarthritis. Osteoarthritis Cartilage. 19(2): 206-12.
  • Topoisomerase inhibitors promote cancer cell motility via ROS-mediated activation of JAK2-STAT1-CXCL1 pathway
    Author
    Liu, J;Qu, L;Meng, L;Shou, C;
    Year
    2019
    Journal
    J. Exp. Clin. Cancer Res.
    Application
    WB

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Mouse MAb

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