Recombinant Anti-Cystatin S Antibody, Rabbit Monoclonal

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Recombinant Anti-Cystatin S Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-Cystatin S Antibody, Rabbit Monoclonal
Validated applications
ELISA,FCM,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human Cystatin S
Immunogen
Recombinant Human Cystatin S / CST4 protein (Catalog#11542-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Cystatin S / CST4 (rh Cystatin S / CST4; Catalog#11542-H08H; NP_001890.1; Met 1-Ala 141).
Source
Monoclonal Rabbit IgG Clone #006
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Cystatin S Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
ELISA 1:5000-1:10000
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Cystatin S Antibody, Rabbit Monoclonal Images

Immunofluorescence staining of Human CST4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CST4 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human CST4 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CST4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-Cystatin S Antibody, Rabbit Monoclonal: Synonyms

Anti-CST4 Antibody; Anti-MGC71923 Antibody

Cystatin S Background Information

Cystatin-S, also known as Cystatin-4, Salivary acidic protein 1, Cystatin-SA-III and CST4, is a secreted protein which belongs to thecystatin family. Cystatin-4 / CST4 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in saliva, tears, urine and seminal fluid. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Cystatin-4 / CST4 strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Cystatin-4 / CST4 is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function.
Full Name
cystatin S
References
  • Bobek L.A., et al.,(1991), Human salivary cystatin S. Cloning, sequence analysis, hybridization in situ and immunocytochemistry. Biochem. J. 278:627-635.
  • Saitoh E., et al., (1992), Characterization of two members (CST4 and CST5) of the cystatin gene family and molecular evolution of cystatin genes.Agents Actions 38:340-348.
  • Deloukas P., et al.,(2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.

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