Anti-Cytokeratin 19/KRT19 Antibody, Rabbit Polyclonal

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Anti-Cytokeratin 19/KRT19 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-Cytokeratin 19/KRT19 Antibody, Rabbit Polyclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human Cytokeratin 19/KRT19
Immunogen
A synthetic peptide corresponding to the C-terminus of the human Cytokeratin 19.
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the human Cytokeratin 19. Human Cytokeratin 19 specific IgG was purified by Human Cytokeratin 19 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Cytokeratin 19/KRT19 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:1000-1:5000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Cytokeratin 19/KRT19 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

KRT19 was immunoprecipitated using:

Lane A:0.5 mg Caco-2 Whole Cell Lysate

4 µL anti-KRT19 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-KRT19 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 44 kDa

Observed band size: 40 kDa

Anti-KRT19 rabbit polyclonal antibody at 1:2000 dilution

Lane A: MCF7 Whole Cell Lysate

Lane B: SKBR3 Whole Cell Lysate

Lysates/proteins at 10 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:44 kDa

Observed band size:39 kDa

Anti-Cytokeratin 19/KRT19 Antibody, Rabbit Polyclonal: Synonyms

Anti-CK19 Antibody; Anti-K19 Antibody; Anti-K1CS Antibody

Cytokeratin 19/KRT19 Background Information

Cytokeratin 19 (KRT19) is a cytoplasmic intermediate filament protein, which is responsible for structural rigidity and multipurpose scaffolds. In several cancers, KRT19 is overexpressed and may play a crucial role in tumorigenic transformation.The KRT19 gene encodes cytokeratin 19, an element of the cytoskeleton whose expression is frequently altered in renal cell carcinoma (RCC).KRT19 was identified as one of the key biomarkers for distinguishing cholangiocarcinoma (CCA) and hepatocellular carcinoma.Expression of KRT19 was specifically high in breast cancers and significantly correlated with their invasiveness. Moreover, knockdown of KRT19 led to increased proliferation, migration, invasion, drug resistance, and sphere formation in breast cancer cells via an upregulated NOTCH signaling pathway.
Full Name
keratin 19, type I

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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