Anti-Cytokeratin 5/KRT5 Antibody, Mouse Monoclonal

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Anti-Cytokeratin 5/KRT5 Antibody, Mouse Monoclonal (Mouse Monoclonal antibody) General Information

Product name
Anti-Cytokeratin 5/KRT5 Antibody, Mouse Monoclonal
Validated applications
WB,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human Cytokeratin 5/KRT5
Immunogen
A synthetic peptide corresponding to the center region of the human KRT5
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the human KRT5. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #03
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Cytokeratin 5/KRT5 Antibody, Mouse Monoclonal (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Cytokeratin 5/KRT5 Antibody, Mouse Monoclonal (Mouse Monoclonal antibody) Images

Anti-KRT5 mouse monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:62 kDa

Observed band size:62 kDa

Immunofluorescence staining of KRT5 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti- KRT5 monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Anti-Cytokeratin 5/KRT5 Antibody, Mouse Monoclonal: Synonyms

Anti-CK5 Antibody; Anti-DDD Antibody; Anti-DDD1 Antibody; Anti-EBS2 Antibody; Anti-K5 Antibody; Anti-KRT5A Antibody

Cytokeratin 5/KRT5 Background Information

Keratin 5 is the major type II keratin of the basal cells of epidermis and of other stratified epithelia. With its type I partner, keratin 14, it constitutes a major fraction of the cytoskeleton of the basal cells. Epidermolysis bullosa simplex with mottled pigmentation (EBS-MP) is a rare dermatologic disorder of autosomal dominant inheritance with intraepidermal blistering after minor trauma, reticular hyperpigmentation unrelated to the blistering, nail dystrophy, and mild palmoplantar keratosis. Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex. Recently, a 71C-->T transition in the keratin 5 gene (KRT5) causing a P24L substitution was identified in some patients with EBS-MP.
Full Name
keratin 5, type II

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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