Anti-Cytokeratin 5/KRT5 Antibody, Rabbit Polyclonal

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Anti-Cytokeratin 5/KRT5 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-Cytokeratin 5/KRT5 Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human Cytokeratin 5/KRT5
Immunogen
A synthetic peptide corresponding to the center region of the Human Cytokeratin 5 / KRT5
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Cytokeratin 5 / KRT5, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Cytokeratin 5/KRT5 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ICC-IF 1:300-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Cytokeratin 5/KRT5 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Immunofluorescence staining of KRT5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human KRT5 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

PDIA4 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg MCF-7 Whole Cell Lysate

2 µL anti-PDIA4 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 73 kDa

Observed band size: 73 kDa

Anti-KRT5 rabbit polyclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:62 kDa

Observed band size:55 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Cytokeratin 5/KRT5 Antibody, Rabbit Polyclonal: Synonyms

Anti-CK5 Antibody; Anti-DDD Antibody; Anti-DDD1 Antibody; Anti-EBS2 Antibody; Anti-K5 Antibody; Anti-KRT5A Antibody

Cytokeratin 5/KRT5 Background Information

Keratin 5 is the major type II keratin of the basal cells of epidermis and of other stratified epithelia. With its type I partner, keratin 14, it constitutes a major fraction of the cytoskeleton of the basal cells. Epidermolysis bullosa simplex with mottled pigmentation (EBS-MP) is a rare dermatologic disorder of autosomal dominant inheritance with intraepidermal blistering after minor trauma, reticular hyperpigmentation unrelated to the blistering, nail dystrophy, and mild palmoplantar keratosis. Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex. Recently, a 71C-->T transition in the keratin 5 gene (KRT5) causing a P24L substitution was identified in some patients with EBS-MP.
Full Name
keratin 5, type II

Standard Antibody Development Service

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Rabbit PAb

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Mouse MAb

Rabbit PAb

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