Anti-DDX4 Antibody, Mouse Monoclonal

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Anti-DDX4 Antibody, Mouse Monoclonal (Mouse Monoclonal antibody) General Information

Product name
Anti-DDX4 Antibody, Mouse Monoclonal
Validated applications
IHC-P,ICC/IF
Species reactivity
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Specificity
Human DDX4
Immunogen
A synthetic peptide corresponding to the C-terminus of the human DDX4.
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human DDX4. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #03
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-DDX4 Antibody, Mouse Monoclonal (Mouse Monoclonal antibody) Validated Applications

Application Dilution
IHC-P 1:50-1:200
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-DDX4 Antibody, Mouse Monoclonal (Mouse Monoclonal antibody) Images

Immunochemical staining of human DDX4 in human testis with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).
Immunofluorescence staining of DDX4 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti- DDX4 monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm.

Anti-DDX4 Antibody, Mouse Monoclonal: Synonyms

Anti-VASA Antibody

DDX4 Background Information

DDX4 (DEAD box polypeptide 4), characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is an RNA helicase which is implicated in various cellular processes involving the alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DDX4 is known to be a germ cell-specific protein and is used as a sorting marker of germline stem cells for the production of oocytes. The active vitamin D treatment prohibited the proliferation and invasion of ovarian cancer cells, and the expression level of a germ cell specific marker DEAD (Asp-Glu-Ala-Asp)-box helicase 4 (DDX4), which is overexpressed in ovarian cancer, was downregulated by active vitamin D treatment. Knockdown of DDX4 by siRNA could also suppress the invasive ability of ovarian cancer cells. Therefore, DDX4 may be considered as a diagnostic marker of ovarian cancer, and vitamin D may be a candidate drug for ovarian cancer therapy. That DDX4 is essential for the regulation of germ cell proliferation and differentiation across all three extant mammalian groups-eutherians, marsupials, and monotremes.
Full Name
DEAD (Asp-Glu-Ala-Asp) box polypeptide 4

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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