Recombinant Anti-EGFR Antibody, Rabbit Monoclonal

RRID Number: AB_2860037
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-EGFR Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) General Information

Product name
Recombinant Anti-EGFR Antibody, Rabbit Monoclonal
Validated applications
ELISA,FCM
Species reactivity
Reacts with: Human
Specificity
Human EGFR
Immunogen
Recombinant Human EGFR / HER1 / ErbB1 Protein (Catalog#10001-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645).
Source
Monoclonal Rabbit IgG Clone #E01
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-EGFR Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:5000-1:10000
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-EGFR Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) Images

Flow cytometric analysis of Human EGFR expression on A431 cells. Cells were stained with purified anti-Human EGFR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-EGFR Antibody, Rabbit Monoclonal: Synonyms

Anti-ERBB Antibody; Anti-ERBB1 Antibody; Anti-HER1 Antibody; Anti-mENA Antibody; Anti-NISBD2 Antibody; Anti-PIG61 Antibody

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
Related Pathways
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.

Standard Antibody Development Service

Rabbit MAb

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Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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