Anti-ER alpha Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information
Anti-ER alpha Antibody, Rabbit Polyclonal
Reacts with: Human
Human ER alpha
A synthetic peptide corresponding to the center region of the Human ER alpha/ESR1
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ER alpha/ESR1, and purified by antigen affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence staining of ER alpha/ESR1 in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ER alpha/ESR1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.
Immunochemical staining of human ER alpha/ESR1 in human endometrial cancer (from 2 donors) with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human ER alpha/ESR1 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
An ESR1 gene mutation, which encodes for the estrogen receptor (ER) protein, is one of the potential mechanisms of therapy resistance. The ESR1 mutations result in conformational changes in the ER leading to subsequent estrogen-independent transcriptional activity. The mutations within the estrogen receptor (ER) gene, ESR1, frequently occur in metastatic breast cancer and influence response to hormone therapy. Estrogen receptor alpha (ERalpha) is one of the estrogen hormone-activated transcription factors, which regulates a large number of genes and is involved in the mammary gland development. Expression of ERalpha is considered to be a good indicator for endocrine therapy and breast cancer survival. Loss of ERalpha in breast cancer patients indicates invasiveness and poor prognosis. ESR1 as a direct target of miR-31a-3p and suggest that miR-31a-3p likely contributes to development of estrogen independence, which leads to a more invasive phenotype of breast cancer. Polymorphisms of the estrogen receptor gene alpha (ESR1) are consistently been associated with bone mineral density (BMD) and fracture.
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