Recombinant Anti-GDF3 Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) General Information
Recombinant Anti-GDF3 Antibody, Rabbit Monoclonal
Reacts with: Human
A synthetic peptide corresponding to the center region of the Human GDF-3
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human GDF-3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Rabbit IgG Clone #010
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunochemical staining of human GDF3 in human testis with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).
Immunochemical staining of human GDF3 in human breast carcinoma with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).
Immunofluorescence staining of GDF3 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GDF3 monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
The GDF3 gene plays a fundamental role in embryonic morphogenesis. GDF3 expression is significantly decreased in human breast cancer tissues, and reconstitution of GDF3 in breast cancer may be a potential therapeutic approach to inhibit aggressive growth of breast cancer.
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