Anti-GSTP1/GST3 Antibody, Rabbit Polyclonal

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Anti-GSTP1/GST3 Antibody, Rabbit Polyclonal General Information

Product name
Anti-GSTP1/GST3 Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF,IP
Species reactivity
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Specificity
Human GSTP1/GST3
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human GSTP1
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human GSTP1, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GSTP1/GST3 Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:1500-1:50000
IP 0.1-0.5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GSTP1/GST3 Antibody, Rabbit Polyclonal Images

Immunofluorescence staining of GSTP1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human GSTP1 polyclonal antibody (1:5000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus..

GSTP1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-GSTP1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GSTP1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 24 kDa

Observed band size: 24 kDa

Anti-GSTP1 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:23 kDa

Anti-GSTP1/GST3 Antibody, Rabbit Polyclonal: Synonyms

Anti-DFN7 Antibody; Anti-FAEES3 Antibody; Anti-GST3 Antibody; Anti-GSTP Antibody; Anti-HEL-S-22 Antibody; Anti-PI Antibody

GSTP1/GST3 Background Information

Glutathione S-transferase (GST) family members promote carcinogenesis and cancer progression. GSTP1 levels correlated negatively with tumor size and serum alpha-fetoprotein (AFP) in HCC patients, and higher GSTP1 levels were associated with longer overall survival (OS) and disease-free survival (DFS), the high GSTP1 levels provide a better prognosis through suppression of tumorigenesis in HCC. Dual-testing of HPV and the GSTP1 might prove an effective screening tool for cervical cancer. GSTP1 is highly expressed in various cancerous cells and pre-neoplastic legions, where it is involved in apoptotic resistance or metabolism of several anti-tumor chemotherapeutics. That GSTP1 is a marker of malignant and pre-malignant cells and is a promising target for visualization and drug development. GSTP1 is a largely investigated tissue biomarker in several malignancies such as prostate, breast, lung, and hepatocellular carcinoma with good performances, especially for diagnostic purposes.
Full Name
glutathione S-transferase pi 1

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Mouse MAb

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