Anti-HNF1A Antibody, Rabbit Polyclonal General Information
Anti-HNF1A Antibody, Rabbit Polyclonal
Reacts with: Human
A synthetic peptide corresponding to the center region of the Human HNF-1-alpha / HNF1A
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human HNF-1-alpha / HNF1A, and purified by antigen affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-HNF1A Antibody, Rabbit Polyclonal Images
Immunofluorescence staining of HNF1A in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human HNF1A polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human HNF1A polyclonal antibody and antigen (Figure B) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.
Gene HNF1A is one of the most common causes of MODY diabetes (HNF1A-MODY; MODY3). This gene is polymorphic and more than 1200 pathogenic and non-pathogenic HNF1A variants were described in its UTRs, exons, and introns. For HNF1A-MODY, not just gene but also phenotype heterogeneity is typical. The transcription factor HNF1A is a putative central regulator of PCSC function. Levels of HNF1A and its target genes were found to be elevated in PCSCs and tumorspheres, and depletion of HNF1A resulted in growth inhibition, apoptosis, impaired tumorsphere formation, decreased PCSC marker expression, and downregulation of POU5F1/OCT4 expression. Conversely, HNF1A overexpression increased PCSC marker expression and tumorsphere formation in pancreatic cancer cells and drove pancreatic ductal adenocarcinoma (PDA) cell growth. Depletion of HNF1A in xenografts impaired tumor growth and depleted PCSC marker-positive cells in vivo.
HNF1 homeobox A
Yu Y, et al. (2019) Hnf1a/casc2 regulates pancreatic cancer cell proliferation through pten/akt signaling. J Cell Biochem 120 (3): 2816-2827.
Valkovicova T, et al. (2019) Novel insights into genetics and clinics of the hnf1a-mody. Endocr Regul 53 (2): 110-134.
Abel EV, et al. (2018) Hnf1a is a novel oncogene that regulates human pancreatic cancer stem cell properties. Elife 7: e33947.
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