Anti-HtrA2/Omi Antibody, Rabbit Polyclonal

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Anti-HtrA2/Omi Antibody, Rabbit Polyclonal General Information

Product name
Anti-HtrA2/Omi Antibody, Rabbit Polyclonal
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human HtrA2/Omi
Immunogen
Recombinant Human HtrA2 Protein (Catalog#10619-H08E)
Preparation
Produced in rabbits immunized with purified, recombinant Human HtrA2 (rh HtrA2; Catalog#10619-H08E; O43464-1; Ala134-Glu458). HtrA2 specific IgG was purified by Human HtrA2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-HtrA2/Omi Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:2000-1:10000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-HtrA2/Omi Antibody, Rabbit Polyclonal Images

Immunochemical staining of human HTRA2 in human kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

HTRA2 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

0.5 µL anti-HTRA2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HTRA2 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 37 kDa

Observed band size: 37 kDa

Anti-HTRA2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HL60 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:49 kDa

Observed band size:37 kDa

Anti-HtrA2/Omi Antibody, Rabbit Polyclonal: Synonyms

Anti-OMI Antibody; Anti-PARK13 Antibody; Anti-PRSS25 Antibody

HtrA2/Omi Background Information

Serine protease HTRA2, also known as high-temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI and HTRA2, is a single-pass membrane protein that belongs to the peptidase S1B family. HTRA2 contains one PDZ (DHR) domain. HTRA2 is a serine protease that shows proteolytic activity against a non-specific substrate beta-casein. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity or by a BIRC inhibition-independent, caspase-independent, and serine protease activity-dependent mechanism. HTRA2 cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 of HTRA2 seems to be proteolytically inactive. Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) which is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity, and postural instability, as well as by a clinically significant response to treatment with levodopa.
Full Name
HtrA serine peptidase 2
References
  • Faccio L., et al., 2000, J. Biol. Chem. 275:2581-2588.
  • Gray C.W., et al., 2000, Eur. J. Biochem. 267:5699-5710.
  • Suzuki Y., et al., 2001, Mol. Cell 8:613-621.
  • Strauss K.M., et al., 2005, Hum. Mol. Genet. 14:2099-2111.

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