Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal

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Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human IFITM1/CD225
Immunogen
A synthetic peptide corresponding to the N-terminus of the human CD225
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the human CD225, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IHC-P 1:10000-1:50000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Immunochemical staining of human IFITM1 in human tonsil with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).

CD225 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg K562 Whole Cell Lysate

0.5 µL anti-CD225 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD225 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 15 kDa

Observed band size: 15 kDa

Anti-IFITM1 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:14 kDa

Observed band size:15 kDa

Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal: Synonyms

Anti-42274 Antibody; Anti-CD225 Antibody; Anti-DSPA2a Antibody; Anti-IFI17 Antibody; Anti-LEU13 Antibody

IFITM1/CD225 Background Information

Full Name
interferon induced transmembrane protein 1

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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