Anti-KLF4 Antibody, Rabbit Polyclonal

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Anti-KLF4 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-KLF4 Antibody, Rabbit Polyclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Specificity
Human KLF4
Immunogen
A synthetic peptide corresponding to the N-terminus of the human KLF4
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the human KLF4, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-KLF4 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IP 1-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-KLF4 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-KLF4 rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lane B: Caco-2 Whole Cell Lysate

Lane C: HEK293 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:54 kDa

Observed band size:65 kDa

KLF4 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-KLF4 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-KLF4 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 65 kDa

Observed band size: 65 kDa

Anti-KLF4 Antibody, Rabbit Polyclonal: Synonyms

Anti-EZF Antibody; Anti-GKLF Antibody

KLF4 Background Information

KLF4 is a well-known transcription factor regulating the expression of several genes including hTERT.KLF4 is a zin-finger transcription factor that plays roles in differentiation, development, and proliferation. KLF4 functions both as a tumor suppressor and oncogene depending on cell type. Kruppel like factor 4 (KLF4), a transcription factor associated with carcinogenesis and tumor progression, plays an important role in various malignancies.Kruppel-like factor 4 (KLF4), a stemness-associated transcription factor, promotes the transcription of miR-7 to repress its own translation so that a KLF4-miR-7 auto-regulatory feedback loop is established for mutual regulation of their expression.
Full Name
Kruppel-like factor 4 (gut)

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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