Anti-CD85i rabbit polyclonal antibody at 1:500 dilution.
Lane A: MCF7 Membrane LysateLysates/proteins at 30 ug per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:53 kDa
Observed band size:53 kDa
CD85i was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate2 µL anti-CD85i rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CD85i rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 53 kDa
Observed band size: 70 kDa