Anti-NSE/ENO2 Antibody, Rabbit Polyclonal

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Anti-NSE/ENO2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-NSE/ENO2 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
Specificity
Human NSE/ENO2
Immunogen
A synthetic peptide corresponding to the N-terminus of the human NSE(Gamma-enolase)
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the human NSE(Gamma-enolase) , and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-NSE/ENO2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IHC-P 1:5000-1:20000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-NSE/ENO2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Immunochemical staining of human NSE in human pancreas with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human NSE in human brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).

Anti-NSE(Gamma-enolase) rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:47 kDa

Observed band size:55 kDa

Anti-NSE/ENO2 Antibody, Rabbit Polyclonal: Synonyms

Anti-HEL-S-279 Antibody; Anti-NSE Antibody

NSE/ENO2 Background Information

The combination of silencing ENO2 and 2-deoxyglucose (2-DG) synergistically inhibited leukemia cell survival. ENO2 may be a biological marker for monitoring chemotherapeutic efficacy and relapse in ALL. Reduced ENO2 expression may be a biomarker for a subset of autistic children. Neuron specific enolase (ENO2, gamma-enolase) has been used as a biomarker to help identify neuroendocrine differentiation in breast cancer.
Full Name
enolase 2 (gamma, neuronal)
References
  • Pechumer H, et al. (1994) Detection of neuron-specific gamma-enolase messenger ribonucleic acid in normal human leukocytes by polymerase chain reaction amplification with nested primers. Lab Invest. 69 (6): 743-9.
  • Craig SP, et al. (1991) Localisation of neurone-specific enolase (ENO2) to 12p13. Cytogenet Cell Genet. 54 (1-2): 71-3.
  • Muley T, et al. (2003) Technical performance and diagnostic utility of the new Elecsys neuron-specific enolase enzyme immunoassay. Clin Chem Lab Med. 41 (1): 95-103.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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