Anti-PDX1 Antibody, Rabbit Polyclonal

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Anti-PDX1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-PDX1 Antibody, Rabbit Polyclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human PDX1
Immunogen
A synthetic peptide corresponding to the N-terminus of the human PDX1
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the human PDX1, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PDX1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PDX1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-PDX1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: Caco-2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:30 kDa

Observed band size:38 kDa

(We are unsure as to the identity of these extra bands.)

PDX1 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-PDX1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-PDX1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 38 kDa

Observed band size: 38 kDa

Anti-PDX1 Antibody, Rabbit Polyclonal: Synonyms

Anti-GSF Antibody; Anti-IDX-1 Antibody; Anti-IPF1 Antibody; Anti-IUF1 Antibody; Anti-MODY4 Antibody; Anti-PAGEN1 Antibody; Anti-PDX-1 Antibody; Anti-STF-1 Antibody

PDX1 Background Information

Pancreatic duodenum homeobox protein-1 (PDX1) is a master regulatory gene in pancreatic development.Pancreatic and duodenal homeobox 1 (PDX1), which is a homeodomain-containing transcription factor and a key regulator for insulin gene expression, beta cell maturation and proper beta cell function maintenance in the pancreas.PDX1 (pancreatic and duodenal homeobox 1) is overexpressed in pancreatic cancer, and its reduction results in tumor regression.
Full Name
pancreatic and duodenal homeobox 1

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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