Recombinant Anti-SPARCL1 Antibody, Rabbit Monoclonal

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Recombinant Anti-SPARCL1 Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) General Information

Product name
Recombinant Anti-SPARCL1 Antibody, Rabbit Monoclonal
Validated applications
ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human SPARCL1
Immunogen
Recombinant Human SPARCL1 / SPARC-like 1 protein (Catalog#10046-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SPARCL1 / SPARC-like 1 (rh SPARCL1 / SPARC-like 1; Catalog#10046-H08H; NP_004675.3; Met1-Phe664).
Source
Monoclonal Rabbit IgG Clone #390
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-SPARCL1 Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-SPARCL1 Antibody, Rabbit Monoclonal (Rabbit Monoclonal antibody) Images

Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SPARCL1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.

Recombinant Anti-SPARCL1 Antibody, Rabbit Monoclonal: Synonyms

Anti-MAST 9 Antibody; Anti-MAST9 Antibody; Anti-PIG33 Antibody; Anti-SC1 Antibody; Anti-SPARCL1 Antibody

SPARCL1 Background Information

SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. In addition, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS).
Full Name
SPARC like 1
References
  • Sullivan MM, et al. (2004) Hevin/SC1, a matricellular glycoprotein and potential tumor-suppressor of the SPARC/BM-40/Osteonectin family. Int J Biochem Cell Biol. 36(6): 991-6.
  • Esposito I, et al. (2007) Tumor-suppressor function of SPARC-like protein 1/Hevin in pancreatic cancer. Neoplasia. 9(1): 8-17.
  • Weimer JM, et al. (2008) A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system. Glia. 56(9): 935-41.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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