Anti-Synapsin 1 Antibody, Rabbit Polyclonal

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Anti-Synapsin 1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-Synapsin 1 Antibody, Rabbit Polyclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Specificity
Human Synapsin 1
Immunogen
A synthetic peptide corresponding to the C-terminus of the human SYN1
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human SYN1, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Synapsin 1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Synapsin 1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

SYN1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg A549 Whole Cell Lysate

2 µL anti-SYN1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SYN1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 74 kDa

Observed band size: 105 kDa

Anti-SYN1 rabbit polyclonal antibody at 1:500 dilution.

Lane A: Mouse brain tissue lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:74 kDa

Observed band size:74 kDa

Anti-Synapsin 1 Antibody, Rabbit Polyclonal: Synonyms

Anti-RP1-230G1.2 Antibody; Anti-SYN1a Antibody; Anti-SYN1b Antibody; Anti-SYNI Antibody

Synapsin 1 Background Information

Synapsin I (SynI) is a synaptic vesicle (SV) phosphoprotein playing multiple roles in synaptic transmission and plasticity by differentially affecting crucial steps of SV trafficking in excitatory and inhibitory synapses. SYN1 in the pathogenesis of non-syndromic intellectual disability, showing that alterations of synaptic vesicle trafficking are one possible cause of this disease, and suggest that distinct mutations in SYN1 may lead to distinct brain pathologies.
Full Name
synapsin I
Related Pathways
  • G Protein-coupled Receptors Signaling
    G Protein-coupled Receptors Signaling

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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