Anti-TrkC Antibody, Mouse Monoclonal

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Anti-TrkC Antibody, Mouse Monoclonal (Mouse Monoclonal antibody) General Information

Product name
Anti-TrkC Antibody, Mouse Monoclonal
Validated applications
ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human TrkC
Has cross-reactivity in ELISA with
Mouse TrkC
No cross-reactivity in ELISA with
Human TrkA/NTRK1
Human TrkB
Immunogen
Recombinant Human TrkC / NTRK3 protein (Catalog#10048-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TrkC / NTRK3 (rh TrkC / NTRK3; Catalog#10048-H08H; NP_001007157.1; Met1-Asp428). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #03
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-TrkC Antibody, Mouse Monoclonal (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-TrkC Antibody, Mouse Monoclonal (Mouse Monoclonal antibody) Images

Immunofluorescence staining of Human TrKC in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human TrKC monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.

Anti-TrkC Antibody, Mouse Monoclonal: Synonyms

Anti-gp145(trkC) Antibody; Anti-TRKC Antibody

TrkC Background Information

NT-3 growth factor receptor also known as neurotrophic tyrosine kinase receptor type 3 or TrkC tyrosine kinase or Trk-C receptor, is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC/NTRK3 is widely expressed in the developing and adult nervous system. In later embryonic development, TrkC/NTRK3 is expressed in various structures of the CNS including the caudatoputamen, septal nuclei, cerebellum, and brainstem. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. In the PNS, trkC hybridization appears to correlate, both temporally and spatially, with the outgrowth of axons toward their peripheral targets. TrkC/NTRK3 is widely expressed in the three identified branches of the mammalian nervous system and appears to correlate with the expression of NT-3, its cognate ligand. The apparent colocalization of trkC transcripts with NT-3 raises the possibility this neurotrophin exerts its trophic effects by a paracrine and/or autocrine mechanism. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in TrkC encoding gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
Full Name
neurotrophic tyrosine kinase, receptor, type 3
References
  • Tessarollo L, et al. (1993) trkC, a receptor for neurotrophin-3, is widely expressed in the developing nervous system and in non-neuronal tissues. Development. 118(2): 463-75.
  • Lamballe F, et al. (1994) Developmental expression of trkC, the neurotrophin-3 receptor, in the mammalian nervous system. J Neurosci. 14(1): 14-28.
  • Klein R, et al. (1994) Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements. Nature. 368(6468): 249-51.

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