Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal

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Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal
Validated applications
ELISA,FCM,ICC/IF
Species reactivity
Reacts with: Mouse
Specificity
Mouse FCGR4
Has cross-reactivity in ELISA with
Human CD64 / FCGR1A
No cross-reactivity in ELISA with
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Immunogen
Recombinant Mouse FCGR4 protein (Catalog#50036-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FCGR4 (rM FCGR4; Catalog#50036-M08H; NP_653142.2; Met 1-Gln 203).
Source
Monoclonal Rabbit IgG Clone #012
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
ELISA 1:5000-1:10000
ICC-IF 1:50-1:1000
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal Images

Immunofluorescence staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow cytometric analysis of FCGR4 expression on Raw264.7 cells. Mouse leukaemic monocyte macrophage cell line RAW 264.7 were stained with anti-mouse FCGR4 (50036-R012), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.

Recombinant Anti-FCGR4 Antibody, Rabbit Monoclonal: Synonyms

Anti-4833442P21Rik Antibody; Anti-CD16-2 Antibody; Anti-FcgammaRIV Antibody; Anti-Fcgr3a Antibody; Anti-FcgRIV Antibody; Anti-Fcrl3 Antibody

FCGR4 Background Information

Full Name
Fc receptor, IgG, low affinity IV
Research Areas
  • A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis
    Author
    Arandjelovic, S;Perry, JSA;Lucas, CD;Penberthy, KK;Kim, TH;Zhou, M;Rosen, DA;Chuang, TY;Bettina, AM;Shankman, LS;Cohen, AH;Gaultier, A;Conrads, TP;Kim, M;Elliott, MR;Ravichandran, KS;
    Year
    2019
    Journal
    Nat. Immunol.
    Application
    FCM

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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