Anti-IL-12 p70/IL12A Antibody, Rabbit Polyclonal

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Anti-IL-12 p70/IL12A Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-IL-12 p70/IL12A Antibody, Rabbit Polyclonal
Validated applications
WB
Species reactivity
Reacts with: Mouse
Specificity
Mouse IL-12 p70/IL12A
Immunogen
A synthetic peptide corresponding to the N-terminus of the mouse IL12A .
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the N-terminus of the mouse IL12A . Mouse IL12A specific IgG was purified by Mouse IL12A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IL-12 p70/IL12A Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IL-12 p70/IL12A Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-IL12A rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH-3T3 Whole Cell Lysate

Lane B: Hu-T78 Whole Cell Lysate

Lane C: Raw264.7 Whole Cell Lysate

Lane D: MDA-MB-231 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:25 kDa

Observed band size:28 kDa

(We are unsure as to the identity of these extra bands.)

Anti-IL-12 p70/IL12A Antibody, Rabbit Polyclonal: Synonyms

Anti-Il-12a Antibody; Anti-IL-12p35 Antibody; Anti-Ll12a Antibody; Anti-p35 Antibody

IL-12 p70/IL12A Background Information

Interleukin-12 subunit alpha (IL12A/IL-12p35) is also known as Cytotoxic lymphocyte maturation factor 35 kDa subunit, cytotoxic lymphocyte maturation factor 1, p35, NK cell stimulatory factor chain 1, and interleukin-12 alpha chain. IL12A/IL-12p35 is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 4-kD subunit that is a member of the cytokine receptor family. IL12A/IL-12p35 is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. In clinical, IL-12 remains a very promising immunotherapeutic agent because recent cancer vaccination studies in animal models and humans have demonstrated its powerful adjuvant properties. The immune modulating characteristics of IL-12 considered responsible for the adjuvant effects, as well as the results of animal and human cancer vaccination studies with IL-12 applied as an adjuvant. IL12A/IL-12p35 indicates a cytokine which is important in the development of prostate cancer.
Full Name
interleukin 12A
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
References
  • Sattler HP, et al. (2000) Novel amplification unit at chromosome 3q25-q27 in human prostate cancer. Prostate. 45(3): 207-15.
  • Lamont AG, et al. (1996) IL-12: a key cytokine in immune regulation. Immunol Today. 17(5): 214-7.
  • Portielje JE, et al. (2003) IL-12: a promising adjuvant for cancer vaccination. Cancer Immunol Immunother. 52(3): 133-44.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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