Anti-MYH7 Antibody, Rabbit Polyclonal

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Anti-MYH7 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-MYH7 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human, Cynomolgus
Specificity
Human MYH7
Immunogen
E. coli-derived Human MYH7 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human MYH7 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-MYH7 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
IP IP:5-10 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-MYH7 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-MYH7 rabbit polyclonal antibody at 1:500 dilution

Lane A: U-251 MG Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:223 kDa

Observed band size:250 kDa

Immunochemical staining of human MYH7 in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human MYH7 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

MYH7 was immunoprecipitated using:

Lane A:0.5 mg Rat skeletal muscle Whole Cell Lysate

4 µL anti-MYH7 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-MYH7 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 223 kDa

Observed band size :223 kDa

Anti-MYH7 Antibody, Rabbit Polyclonal: Synonyms

Anti-CMD1S Antibody; Anti-CMH1 Antibody; Anti-MPD1 Antibody; Anti-MYHCB Antibody; Anti-SPMD Antibody; Anti-SPMM Antibody

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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