Anti-NAA10/ARD1 Antibody, Rabbit Polyclonal

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Anti-NAA10/ARD1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-NAA10/ARD1 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,ICC/IF,IP
Species reactivity
Reacts with: Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Specificity
Human NAA10/ARD1
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human ARD1A/NAA10
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ARD1A/NAA10, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-NAA10/ARD1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:100-1:400
ICC-IF 1:50-1:200
IP IP:5-10 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-NAA10/ARD1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-NAA10 rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH-3T3 Whole Cell Lysate

Lane B: RAW264.7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:27 kDa

Immunochemical staining of human NAA10 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human NAA10 in human prostate with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of NAA10 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NAA10 polyclonal antibody (dilution ratio 1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

NAA10 was immunoprecipitated using:

Lane A:0.5 mg NIH 3T3 Whole Cell Lysate

4 µL anti-NAA10 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-NAA10 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 26 kDa

Observed band size :28 kDa

Anti-NAA10/ARD1 Antibody, Rabbit Polyclonal: Synonyms

Anti-ARD1 Antibody; Anti-ARD1A Antibody; Anti-ARD1P Antibody; Anti-DXS707 Antibody; Anti-MCOPS1 Antibody; Anti-NAA10 Antibody; Anti-NATD Antibody; Anti-OGDNS Antibody; Anti-TE2 Antibody

NAA10/ARD1 Background Information

ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.
Full Name
N(alpha)-acetyltransferase 10, NatA catalytic subunit
References
  • Tribioli C., et al.,(1994), Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae. Hum. Mol. Genet. 3:1061-1068.
  • Arnesen T., et al., (2005), Identification and characterization of the human ARD1-NATH protein acetyltransferase complex.Biochem. J. 386:433-443.
  • Ross M.T., et al.,(2005), The DNA sequence of the human X chromosome.Nature 434:325-337.

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