Anti-NCAPH Antibody, Rabbit Polyclonal

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Anti-NCAPH Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-NCAPH Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human NCAPH
Immunogen
E. coli-derived Human NCAPH fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human NCAPH fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-NCAPH Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:250-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-NCAPH Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution

Lane A: HeLa Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:83 kDa

Immunofluorescence staining of NCAPH in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NCAPH polyclonal antibody (dilution ratio 1:500) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.

Anti-NCAPH Antibody, Rabbit Polyclonal: Synonyms

Anti-BRRN1 Antibody; Anti-CAP-H Antibody

NCAPH Background Information

Non-SMC condensin I complex subunit H (NCAPH) in colon cancerous tissues was higher than that in all corresponding adjacent non-cancerous tissues.NCAPH is highly expressed in colorectal cancer cell lines comparing with normal human colonic epithelial cells, and identified many NCAPH mutations in colon cancer (CC) patients.Depletion of NCAPH inhibits CC cell proliferation, migration in vitro and xenograft tumor formation in vivo.NCAPH knockdown promotes cell apoptosis and cell cycle arrest at G2/M phase.NCAPH could be used as a new therapeutic target in CC in future.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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