Anti-OXCT1 Antibody, Rabbit Polyclonal

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Anti-OXCT1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-OXCT1 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human OXCT1
Immunogen
E. coli-derived Human OXCT1 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human OXCT1 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-OXCT1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:200-1:1000
ICC-IF 1:100-1:500
IP IP:1-5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-OXCT1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-OXCT1 rabbit polyclonal antibody at 1:500 dilution

Lane A: U-251 MG Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:56 kDa

Observed band size:55 kDa

Immunochemical staining of human OXCT1 in human stomach with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of OXCT1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human OXCT1 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

OXCT1 was immunoprecipitated using:

Lane A:0.5 mg U251MG Whole Cell Lysate

4 µL anti-OXCT1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-OXCT1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 56 kDa

Observed band size :56 kDa

Anti-OXCT1 Antibody, Rabbit Polyclonal: Synonyms

Anti-OXCT Antibody; Anti-SCOT Antibody

OXCT1 Background Information

Full Name
3-oxoacid CoA transferase 1

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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