Anti-PAX2 Antibody, Rabbit Polyclonal

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Anti-PAX2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-PAX2 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,ICC/IF,IP
Species reactivity
Reacts with: Human, Mouse
Specificity
Human PAX2
Immunogen
A synthetic peptide corresponding to the center region of the Human PAX2
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PAX2, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PAX2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:500-1:2000
ICC-IF 1:100-1:500
IP IP:0.5-2μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PAX2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-PAX2 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:44 kDa

Observed band size:44 kDa

Immunochemical staining of human PAX2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human PAX2 in human renal carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human PAX2 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of PAX2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PAX2 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .

CBX4 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

4 µL anti-CBX4 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-CBX4 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 44 kDa

Observed band size :45 kDa

Anti-PAX2 Antibody, Rabbit Polyclonal: Synonyms

Anti-PAPRS Antibody; Anti-RP11-179B2.1 Antibody

PAX2 Background Information

PAX2 (paired box gene 2) is a transcription factor, which is involved in both cell proliferation and carcinogenesis. PAX2 is one of nine PAX genes that regulate tissue development and cellular differentiation in embryos.PAX2, a nuclear transcription factor in renal development, is strongly expressed during kidney development and plays an important role in kidney development, differentiation, and renal cell proliferation. Paired box (PAX) 2, a transcription factor, plays a critical role in embryogenesis. When aberrantly expressed in adult tissues, it generally exhibits oncogenic properties.PAX2 is expressed in epithelial cells of fetal kidneys and its expression is up-regulated in certain pathologic conditions.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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