Anti-PDLIM1 Antibody, Rabbit Polyclonal

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Anti-PDLIM1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-PDLIM1 Antibody, Rabbit Polyclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human PDLIM1
Immunogen
E. coli-derived Human PDLIM1 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human PDLIM1 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-PDLIM1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IP IP:0.5-2μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PDLIM1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-PDLIM1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: HeLa Whole Cell Lysate

Lane C: 293T Whole Cell Lysate

Lane D: Caco-2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:36 kDa

PDLIM1 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

4 µL anti-PDLIM1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-PDLIM1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 36 kDa

Observed band size :36 kDa

Anti-PDLIM1 Antibody, Rabbit Polyclonal: Synonyms

Anti-CLIM1 Antibody; Anti-CLP-36 Antibody; Anti-CLP36 Antibody; Anti-hCLIM1 Antibody; Anti-HEL-S-112 Antibody

PDLIM1 Background Information

Full Name
PDZ and LIM domain 1

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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