Anti-PRNP/Prion Protein Antibody, Rabbit Polyclonal

1/2
Price:
Size:
Number:

Anti-PRNP/Prion Protein Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-PRNP/Prion Protein Antibody, Rabbit Polyclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human PRNP/Prion Protein
Immunogen
E. coli-derived Human PrP/PRNP fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human PrP/PRNP fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-PRNP/Prion Protein Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IP IP:1-2μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PRNP/Prion Protein Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-PRNP rabbit polyclonal antibody at 1:500 dilution

Lane A: HEK293 Whole Cell Lysate

Lane B: U-251MG Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:28 kDa

PRNP was immunoprecipitated using:

Lane A:0.5 mg U-251MG Whole Cell Lysate

1 µL anti-PRNP rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-PRNP rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size :28 kDa

Anti-PRNP/Prion Protein Antibody, Rabbit Polyclonal: Synonyms

Anti-AltPrP Antibody; Anti-ASCR Antibody; Anti-CD230 Antibody; Anti-CJD Antibody; Anti-GSS Antibody; Anti-KURU Antibody; Anti-p27-30 Antibody; Anti-PRIP Antibody; Anti-PrP Antibody; Anti-PrP27-30 Antibody; Anti-PrP33-35C Antibody; Anti-PrPc Antibody

PRNP/Prion Protein Background Information

Prion protein (PRNP) gene is well known for affecting mammal transmissible spongiform encephalopathies (TSE), and is also reported to regulate phenotypic traits (e.g. growth traits) in healthy ruminants. PRNP is fundamental in the pathogenesis of transmissible spongiform encephalopathies. Generally, PRNP gene is associated with prion diseases, whereas variants of the PRNP gene may also explain some cases of Alzheimer disease (AD) and frontotemporal dementia (FTD) in Caucasian populations.
Full Name
prion protein

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.