Anti-PYGO2/Pygopus-2 Antibody, Rabbit Polyclonal

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Anti-PYGO2/Pygopus-2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-PYGO2/Pygopus-2 Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human PYGO2/Pygopus-2
Immunogen
A synthetic peptide corresponding to the center region of the Human Pygopus-2/PYGO2
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Pygopus-2/PYGO2, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PYGO2/Pygopus-2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:500-1:2500
IP IP:1-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PYGO2/Pygopus-2 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-PYGO2 rabbit polyclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lane B: HeLa Whole Cell Lysate

Lane C: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:41 kDa

Observed band size:50 kDa

Immunofluorescence staining of PYGO2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PYGO2 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.

PYGO2 was immunoprecipitated using:

Lane A:0.5 mg MCF7 Whole Cell Lysate

1 µL anti-PYGO2 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-PYGO2 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 41 kDa

Observed band size :54 kDa

Anti-PYGO2/Pygopus-2 Antibody, Rabbit Polyclonal: Synonyms

Anti-1190004M21Rik Antibody; Anti-PP7910 Antibody

PYGO2/Pygopus-2 Background Information

PYGO2 overexpression enhances primary tumor growth and local invasion to draining lymph nodes. Conversely, PYGO2 depletion inhibits prostate cancer cell invasion in vitro and progression of primary tumor and metastasis in vivo In clinical samples, PYGO2 upregulation associated with higher Gleason score and metastasis to lymph nodes and bone. Silencing PYGO2 expression in patient-derived xenograft models impairs tumor progression. Finally, PYGO2 is necessary to enhance the transcriptional activation in response to ligand-induced Wnt/beta-catenin signaling.YGO2 functions as a driver oncogene in the 1q21.3 amplicon and may serve as a potential prognostic biomarker and therapeutic target for metastatic prostate cancer.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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