Anti-TPK1 Antibody, Rabbit Polyclonal

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Anti-TPK1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-TPK1 Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human TPK1
Immunogen
E. coli-derived Human TPK1 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human TPK1 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-TPK1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:500-1:2500
IP IP:0.2-1μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-TPK1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-TPK1 rabbit polyclonal antibody at 1:500 dilution

Lane A: SH-SY5Y Whole Cell Lysate

Lane B: H1299 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:27 kDa

Immunofluorescence staining of TPK1 in PC3 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human TPK1 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

TPK1 was immunoprecipitated using:

Lane A:0.5 mg H1299 Whole Cell Lysate

1 µL anti-TPK1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-TPK1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 29 kDa

Observed band size :30 kDa

Anti-TPK1 Antibody, Rabbit Polyclonal: Synonyms

Anti-HTPK1 Antibody; Anti-PP20 Antibody; Anti-THMD5 Antibody

TPK1 Background Information

Thiamine pyrophosphokinase (TPK) produces thiamine pyrophosphate, a cofactor for a number of enzymes, including pyruvate dehydrogenase and 2-ketoglutarate dehydrogenase. Episodic encephalopathy type thiamine metabolism dysfunction (OMIM 614458) due to TPK1 mutations is a recently described rare disorder. The genomic variations in the fetal and maternal TPK1 gene could contribute to the variability of birth weight in normal humans.
Full Name
thiamin pyrophosphokinase 1

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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