Anti-TUFM Antibody, Rabbit Polyclonal

Price:
Size:
Number:

Anti-TUFM Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-TUFM Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human TUFM
Immunogen
E. coli-derived Human TUFM fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human TUFM fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-TUFM Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
ICC-IF 1:100-1:500
IP IP:1-5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-TUFM Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-TUFM rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: 431 Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lane D: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:48 kDa

Immunochemical staining of human TUFM in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of TUFM in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TUFM polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

TUFM was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

4 µL anti-TUFM rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-TUFM rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 50 kDa

Observed band size :50 kDa

Anti-TUFM Antibody, Rabbit Polyclonal: Synonyms

Anti-COXPD4 Antibody; Anti-EF-TuMT Antibody; Anti-EFTU Antibody; Anti-P43 Antibody

TUFM Background Information

Full Name
Tu translation elongation factor, mitochondrial

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.