Anti-UBAP1 Antibody, Rabbit Polyclonal

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Anti-UBAP1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-UBAP1 Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human UBAP1
Immunogen
E. coli-derived Human UBAP1 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human UBAP1 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-UBAP1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:2000-1:10000
IHC-P 1:50-1:200
ICC-IF 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-UBAP1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-UBAP1 rabbit polyclonal antibody at 1:2000 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: U251MG Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:60 kDa

Immunochemical staining of human UBAP1 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human UBAP1 in human large intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of UBAP1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human UBAP1 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Anti-UBAP1 Antibody, Rabbit Polyclonal: Synonyms

Anti-NAG20 Antibody; Anti-UAP Antibody; Anti-UBAP Antibody; Anti-UBAP-1 Antibody

UBAP1 Background Information

Full Name
ubiquitin associated protein 1

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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